Glass slides scanned on various devices (Leica's Aperio GT450, AT2, and ScanScope XT Philips UltraFast Scanner Hamamatsu's Nanozoomer 2.0HT and 3DHistech's P1000) were compared to acquired digital slides reviewed on each vendor's respective WSI viewer software (e.g., Aperio ImageScope, ImageScope DX, Philips IMS, 3DHistech CaseViewer, and Hamamatsu NDP.view) and an in-house developed vendor-agnostic viewer. The aim of this study was to determine the parameters that influence HPF in whole slide images (WSIs). In the era of digital pathology, new variables are introduced that may affect HPF evaluation. With traditional light microscopy, HPFs are typically evaluated by quantifying histologic events in 10 fields of view at × 400 magnification.
The microscope high-power field (HPF) is the cornerstone for histopathology diagnostic evaluation such as the quantification of mitotic figures, lymphocytes, and tumor grading.
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